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ATP synthase

ATP synthase is a membrane-bound enzyme that uses the flow of protons (H+) across a membrane to drive the synthesis of ATP from ADP and phosphate. It is found in mitochondria and chloroplasts, and plays a crucial role in cellular energy production and photosynthesis.

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  • blobby green style avatar for user Asbjørn Ravn Rasmussen
    I am really trying hard to find somewhere that tells me excactly how many H+ transfers is needed for resynthase of ATP? In my Sportsscience class at Aarhus University we're taught 1 H+ generates energy for 1 ADT+P -> ATP. I found something very wrong with our entire curriculum around this so I did some digging. At Bsc Medicine at Aarhus Uni they're taught the 'Metabolic Pathways of a Cell' from sigma-aldrich.com/pathways (which I ultimatly trust the most), and they say it's 10 H+ transers for 3 ATP (so roughly 3,3 H+ for 1 ATP). Wikipedia states it's 3 H+ for 1 ATP. And then I've found videos on YouTube stating 4 H+ for one ATP.. What to believe here?
    Many thanks!
    (6 votes)
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    • aqualine ultimate style avatar for user Phiix174
      As per my knowledge ,

      In mitochondria ,

      -1NADH+H+ Produces 3 ATP (3 pairs of H+)

      -1FADH2 produces 2 ATP (2 pairs of H+)

      So basically in mitochondria one pair of H+ produces 1 ATP
      In other words due to movement of 2 protons across the membrane of mitochondria ; conformational change in F1 part results in synthesis of 1 ATP molecule from ADP + Pi

      whereas in chloroplast 3 H+ produce 1 ATP
      That is movement of 3 protons across lumen to stroma through CF1 produce 1 molecule of ATP

      I hope this helps as I know how frustrating such questions can be :)
      (2 votes)
  • mr pink red style avatar for user John
    Is it H+ moving through ATP synthase or is it actually H3O+? I understand how in chemistry the two are used interchangeably. But in this case, is it literally just H+? Thanks!
    (4 votes)
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  • piceratops ultimate style avatar for user Pieter
    What are thylakoids (or lumen) in chloroplasts mainly used for?
    (2 votes)
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  • purple pi teal style avatar for user Joseph
    At , isn't it "mitochondrion"?
    (1 vote)
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Video transcript

- [Instructor] In this video, we're going to talk about what is arguably my favorite enzyme, and that is ATP synthase. And you might be able to predict from its name what it does. It synthesizes ATP. Now you've probably seen it before. We saw it when we looked at respiration, or you will see it when you look at respiration, which is going on in most of the cells of your body. And you also see it when you study photosynthesis. The general thing that it does is, is it sits across a phospholipid membrane. And through other processes, you will have hydrogen ion concentration increase on one side of the membrane, have a higher hydrogen ion concentration on one side than on the other side. You still might have a few over here. And a hydrogen ion is essentially a proton. So on this side of the membrane, it'll be more positive, so there will be a electromotive force to go to the other side. And also, you just have a higher concentration, so there's a chemical gradient, a concentration gradient, where if there was some way for these protons to get to this side, they would want to get there. So there's an electrochemical gradient that they would want to go down. And ATP synthase provides a channel for those protons. But as those protons travel through the ATP synthase, they turn this part of it, which drives this axle, and then this axle nudges these parts of the protein so that they jam together an ADP with a phosphate group to produce ATP. So down here, you, going into this part of the complex, you'll have an ADP and a phosphate group. And then that rotation force that's provided by that electrochemical gradient, that then produces our ATP. And that's going to be the case both in respiration, which occurs in the mitochondria, and in photosynthesis, which occur in chloroplasts. Now there's a few differences. In mitochondria, the hydrogen ions, these protons, the concentration builds up in the intermembrane space right over here because of the electron transport chain. And we studied that in other videos. And then the protons travel through the ATP synthase. You could see a little mini version right over here. You could imagine that what we see really big, that is a blown-up version of this part of the mitochondria, and this is, of course, is not to scale. So in the case of a mitochondria, this would be the inner membrane. Right over here would be the intermembrane space between the inner and the outer membrane, intermembrane space. And right over here would be the matrix of the mitochondria. And so as the protons go through, they're able to produce ATP in the matrix. Now in chloroplasts, the hydrogen protons build up inside the thylakoids, which are these parts of the chloroplast. That space inside the thylakoid is often called the thylakoid space, sometimes called the lumen. That proton buildup inside the thylakoids happens because of the light reactions, the first phase of photosynthesis. But then those protons will travel through the thylakoid membrane, through to this area, which is known as the stroma in chloroplasts, and they produce the ATP in the stroma. But then the ATP is used in the second phase of photosynthesis to synthesize carbohydrates, which is, you could view as one of the end products of photosynthesis. So the big takeaway of this video is, one, ATP synthase is incredibly cool. If you look up on the internet, you can find some simulations that show ATP synthase and how it acts like a motor to jam the phosphate group to the ADP to produce ATP. And ATP synthase in mitochondria and chloroplasts are remarkably similar, although they sit in different parts of these organelles. And the ATP in mitochondria, you can view as the end product of respiration, while the ATP produced in chloroplasts is an intermediary store of energy, which is then used to synthesize carbohydrates.